Preconditioning delays Ca2+-induced mitochondrial permeability transition

Transient mitochondrial permeability transition pore opening mediates preconditioning-induced protection.

BACKGROUND Transient (low-conductance) opening of the mitochondrial permeability transition pore (mPTP) may limit mitochondrial calcium load and mediate mitochondrial reactive oxygen species (ROS) signaling. We hypothesize that transient mPTP opening and ROS mediate the protection associated with myocardial preconditioning and mitochondrial uncoupling. METHODS AND RESULTS Isolated perfused ra...

Mitochondrial Transition Pore and Preconditioning 1 Preconditioning Protects by Inhibiting the Mitochondrial Permeability Transition

Mitochondrial depolarization underlies delay in permeability transition by preconditioning with isoflurane: roles of ROS and Ca2+.

During reperfusion, the interplay between excess reactive oxygen species (ROS) production, mitochondrial Ca(2+) overload, and mitochondrial permeability transition pore (mPTP) opening, as the crucial mechanism of cardiomyocyte injury, remains intriguing. Here, we investigated whether an induction of a partial decrease in mitochondrial membrane potential (DeltaPsi(m)) is an underlying mechanism ...

Calcium preconditioning inhibits mitochondrial permeability transition and apoptosis.

We tested the hypothesis whether calcium preconditioning (CPC) reduces reoxygenation injury by inhibiting mitochondrial permeability transition (MPT). Cultured myocytes were preconditioned by a brief exposure to 1.5 mM calcium (CPC) and subjected to 3 h of anoxia followed by 2 h of reoxygenation (A-R). Myocytes were also treated with 0.2 microM/l cyclosporin A (CsA), an inhibitor of MPT, before...

Ischaemic preconditioning and mitochondrial permeability transition: a long-lasting relationship.

Since its first description 25 years ago, ischaemic preconditioning has become one of the most studied protective interventions in the cardiovascular field, in part due to its universality (it is effective in all animal species tested, including humans, and in different organs) and reproducibility (it has only a weak dependence on the ischaemic protocol applied before sustained ischaemia or on ...